Development of a New Framework for Health Assessment in Patients With Coronary Artery Disease by Using microRNA-197 in Iranian Adults

Document Type : Original Article


1 Department of biology, Faculty of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, IR Iran.

2 Department of Medical Genetics, Kermanshah University of Medical Sciences, Kermanshah, IR Iran.

3 Research and Educational Center, Imam Ali Cardiovascular Hospital, Kermanshah University of Medical Sciences, Kermanshah, IR Iran.


Background: Coronary artery disease (CAD) refers to stenosis or obstruction in a part or all of a coronary artery due to atherosclerosis or clotting. This study aimed to evaluate the possible efficacy of the serum microRNA-197 (miR-197) as an indicator of diagnosis in patients with CAD.
Methods: In this study, 100 patients with CAD who had angiography and vascular transplantation were selected and evaluated. The expression level of miR-197 was determined via the real-time RT-PCR technique and the SYBR Green method. For the analysis of the miRNA expression level and the significance of the patient sample, the t test was used. Additionally, the Pearson correlation coefficient test was utilized to determine the relationship between the expression levels of miRNAs and CAD severity.
Results: A positive correlation was observed between miR-197 expression and CAD severity. The average expression of 0.78 in the control sample was increased to 2.76 according to the severity of involvement in the patient. In other words, the relative expression of miR-197 in the CAD + group was significantly increased compared with the control group (P<0.004).
Conclusions: It appears that miR-197 can be considered an indicator of coronary endothelial cell function, and it is possible to use it as a biomarker for the prognosis, control, or treatment of CAD. (Iranian Heart Journal 2022; 23(1): 17-24)


  1. Kanuri SH, Ipe J, Kassab K, Gao H. Next generation MicroRNA sequencing to identify coronary artery disease patients at risk of recurrent myocardial infarction. Atherosclerosis. 2018 Oct 3;278:232-239. doi: 10.1016/0002-9149(63)90064-X. [PubMed: 16141261].
  2. Mozaffarian, E.J. Benjamin, A.S. Heart disease and stroke Statistics-2016 update: a report from the American heart association. Circulation, 133 (2016) e38-360. doi:10.1016/0002-9343(77)90423-5. [PubMed: 20961243].
  3. Christian Albus, Jörg Barkhausen. The Diagnosis of Chronic Coronary Heart Disease. Dtsch Arztebl Int. 2017 Oct; 114(42):712–719. doi:10.1161/01.CIR.67.1.134.[PubMed: 25205802].
  4. Albus, C; Barkhausen, J . The Diagnosis of Chronic Coronary Heart Disease. Dtsch Arztebl Int 2017; 114(42): 712-9. doi: 10.1161/CIRCULATIONAHA.112.130153. [PubMed: 21639811].
  5. Caterina Catalanotto, Carlo Cogoni. MicroRNA in Control of Gene Expression: An Overview of Nuclear Functions. Int J Mol Sci. 2016 Oct; 17(10): 1712. doi:10.1093/omcr/omw036. [PubMed: 22391581].
  6. Hongyu Liu, Cheng Lei, Qin He. Nuclear functions of mammalian MicroRNAs in gene regulation, immunity and cancer. Molecular Cancer2018; 17:64. doi:10.2353/ajpath.2006.050646. [PubMed Central: PMC4169002].
  7. Mihailescu R. Gene expression regulation: lessons from noncoding RNAs. RNA. 2015 Apr; 21(4):695-6. doi:10.1055/s-2005-872844. [PubMed Central: PMC4060354].
  8. Christian Schulte, Simon Molz, Sebastian Appelbaum. miRNA-197 and miRNA-223 Predict Cardiovascular Death in a Cohort of Patients with Symptomatic Coronary Artery Disease. PLoS One. 2015 Dec 31; 10(12). doi:10.1242/jcs.184770. [PubMed: 15673802].
  9. Lerman G, Sharon M, Leibowitz-Amit R, Sidi Y, Avni D. The crosstalk between IL-22 signaling and miR-197 in human. PLoS kOne. 2014 Sep 10;9(9). doi:10.1097/GIM.0b013e31820ad795. [PubMed: 16565504].
  10. Huamin Wang,Xiaoping Su,Mingjin Yang,Taoyong Chen,Jin Hou,Nan Li. Reciprocal control of miR-197 and IL-6/STAT3 pathway reveals miR-197 as potential therapeutic target for hepatocellular carcinoma. Oncoimmunology. 2015 Jun 4; 4(10):e1031440. doi:10.1001/jama.2009.371. [PubMed: 18377432].
  11. Fiori ME, Barbini C, Haas TL, Marroncelli N, Patrizii M, Biffoni M, De Maria R. Antitumor effect of miR-197 targeting in p53 wild-type lung cancer. Cell Death Differ. 2014 May; 21(5):774-82. doi:10.1002/mus.23517. [PubMed: 16217705].
  12. Legchenko E, Chouvarine P, Borchert P, Fernandez-Gonzalez A. PPARγ agonist pioglitazone reverses pulmonary hypertension and prevents right heart failure via fatty acid oxidation. Sci Transl Med. 2018 Apr 25; 10(438). doi:10.14503/THIJ-13-3896. [PubMed: 21415759].
  13. Jia HL, Liu CW, Zhang L, Xu WJ. Sets of serum exosomal microRNAs as candidate diagnostic biomarkers for Kawasaki disease. Sci Rep. 2017 Mar 20; 7:44706. doi: 10.4103/0189-7969.187732. [PubMed: 12084876].
  14. McManus DD, Rong J, Huan T, Lacey S. Messenger RNA and MicroRNA transcriptomic signatures of cardiometabolic risk factors. BMC Genomics. 2017 Feb 8;18(1):139. doi:10.14503/THIJ-15-5450. [PubMed: 23168931].
  15. Tang T, Cheng Y, She Q, Jiang Y, Chen Y, Yang W, Li Y. Long non-coding RNA TUG1 sponges miR-197 to enhance cisplatin sensitivity in triple negative breast cancer. Biomed Pharmacother. 2018 Nov; 107:338-346. doi:10.1093/eurheartj/ehi471. [PubMed: 22695892].
  16. Lu X, Liu Z, Ning X, Huang L, Jiang B. The Long Noncoding RNA HOTAIR Promotes Colorectal Cancer Progression by Sponging miR-197. Oncol Res. 2018 Apr 10; 26(3):473-481. doi:10.4244/EIJV8I1A20. [PubMed: 28638575].
  17. Zhang Y, Huang S, Li P, Chen Q, Li Y. Pancreatic cancer-derived exosomes suppress the production of GIP and GLP-1 from STC-1 cells in vitro by down-regulating the PCSK1/3. Cancer Lett. 2018 Sep 1;431:190-200. doi:10.3978/j.issn.2225-319X.2015.02.05. [PubMed: 30393640].
  18. Orlicka-Plocka M, Gurda D. Circulating microRNAs in cardiovascular diseases. Acta Biochim Pol.2016;63(4): 725-729. doi: 10.1016/j.jjcc.2015.09.009. [PubMed: 28627787].
  19. Wei-Ping Tien, Gareth Lim. SYBR green-based one step quantitative real-time polymerase chain reaction assay for the detection of Zika virus in field-caught mosquitoes. Parasit Vectors. 2017; 10: 427. doi:10.1002/ajmg.a.38320. [PubMed: 22457381].
  20. Marilia Farignoli, William Marciel. Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil. Rev. Soc. Bras. Med. 2016; 167-198. doi:10.1016/j.spen.2006.06.004. [PubMed: 15977643].