Development of a New Framework for Health Assessment in Patients With Coronary Artery Disease by Using microRNA-197 in Iranian Adults

Document Type : Original Article

Authors

1 Department of biology, Faculty of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, IR Iran.

2 Department of Medical Genetics, Kermanshah University of Medical Sciences, Kermanshah, IR Iran.

3 Research and Educational Center, Imam Ali Cardiovascular Hospital, Kermanshah University of Medical Sciences, Kermanshah, IR Iran.

Abstract

Background: Coronary artery disease (CAD) refers to stenosis or obstruction in a part or all of a coronary artery due to atherosclerosis or clotting. This study aimed to evaluate the possible efficacy of the serum microRNA-197 (miR-197) as an indicator of diagnosis in patients with CAD.
 
Methods: In this study, 100 patients with CAD who had angiography and vascular transplantation were selected and evaluated. The expression level of miR-197 was determined via the real-time RT-PCR technique and the SYBR Green method. For the analysis of the miRNA expression level and the significance of the patient sample, the t test was used. Additionally, the Pearson correlation coefficient test was utilized to determine the relationship between the expression levels of miRNAs and CAD severity.
 
Results: A positive correlation was observed between miR-197 expression and CAD severity. The average expression of 0.78 in the control sample was increased to 2.76 according to the severity of involvement in the patient. In other words, the relative expression of miR-197 in the CAD + group was significantly increased compared with the control group (P<0.004).
 
Conclusions: It appears that miR-197 can be considered an indicator of coronary endothelial cell function, and it is possible to use it as a biomarker for the prognosis, control, or treatment of CAD. (Iranian Heart Journal 2022; 23(1): 17-24)

Keywords

References

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Iranian Heart Journal
Volume 23, Issue 1
January 2022
Pages 17-24

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